Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...
In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.
There are many popular chemistries for real-time PCR. One class uses different fluorescent dyes incorporated in short oligonucleotide probes specific for the amplified target. The second class ...
As K is dependent on factors like (1) the reporter dye used in the probe (2) sequence context ... Thus, the overall efficiency of RT– and real-time PCR in detecting the housekeeping gene in ...
The GoTaq ® Probe 1-Step RT-qPCR System enables detection and relative ... control of possible carryover contamination. Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure ...
Let us help you with your inquiries, brochures and pricing requirements Request A Quote Download PDF Copy Download Brochure The QuantStudio 6 and 7 Pro real-time PCR ...
This innovative kit allows for the rapid detection of bacterial and fungal contamination within a mere 3 hours, leveraging the accuracy and reliability of real-time PCR technology ... High Specificity ...
Expert Rev Mol Diagn. 2012;12(7):731-754. SYBR Green and dual-hybridization probes are often used for melting point analysis at the end of the amplification. qPCR presents a number of advantages ...