Clinical-grade CAR-T cell manufacturing was performed in a closed system using a second-generation CD19 vector with 41BB costimulatory ... by quantitative PCR with the MACS Copycheck Kit human ...

German biotechnology company Miltenyi Biotec has said that its CAR-T therapy ... The company said that ZAMTO Cell is designed to target both CD19 and CD20, which could improve treatment efficacy.

T cells were then isolated using the EasySep human T cell isolation kit (StemCell, Vancouver ... Primary human T cell media was also supplemented with 100 IU/ml rhIL-2 (Miltenyi, Bergisch Gladbach, ...

CD19 + B cells were isolated by human CD19 microbeads (Miltenyi Biotec, USA ... Intracellular staining utilized the fixation/permeabilization kit (BD Biosciences, USA). Cells were suspended in a ...

Axi-cel, tisa-cel, and brexu-cel were found comparable to other cellular therapy experiences for patients with B-cell ...

Although CD19-directed CAR T cells can initially induce remission in 70-90% of patients with relapsed/refractory B-cell acute lymphoblastic leukemia (B-ALL), post-CAR relapses are frequent. These ...