Human umbilical vein endothelial cells (HUVECs) were seeded at 1 × 104 cells per cm2 in six-well plates. Treatment with TNF-α (2.5, 5 or 10 ng/ml) or lipopolysaccharide (0.5, 1 or 5 μg/ml) treatment ...

Based on the flow cytometry markers, fixatives can extend the sample stability several weeks to months. PBMC isolation can also be an alternative solution for high-quality flow cytometry data.