To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right). MeltPlex®utilizes a system of labelled ...

Real-time PCR is the favored method for measuring gene expression. ... Each TaqMan probe consists of a single-stranded oligonucleotide that is complementary to a sequence within the target template.

Species-specific TaqMan® probes were designed to detect six bacterial species. ... Real-time PCR detection assays commonly target VP7 using consensus primers and probes designed to detect all ...

The TaqMan probe is a specific sequence of DNA which acts as the primer for the DNA polymerase. ... Real-Time/Quantitative PCR in Industry. News-Medical, viewed 14 June 2025, ...

These probes were cut using restriction enzymes, in a process called oligomer restriction, ... Real-time PCR and Taqman™ technology were also originally conceived by the PCR team at Cetus.

New highly efficient molecular probe for real-time PCR monitoring and genetic testing. ScienceDaily. Retrieved June 2, 2025 from www.sciencedaily.com / releases / 2013 / 08 / 130807204704.htm.

We present data for TaqMan amplification that demonstrates the ability to detect a 0.8% mutant abundance among wild-type alleles with one round of real-time COLD-PCR (Figure 5D).

In the modified DMA approach, the TaqMan probe is also used during the asymmetric PCR step as a blocking agent for the wild-type allele, ... TaqMan probes as blocking agents for enriched PCR ...

Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on ...

Eprobe, a highly efficient and reliable fluorescent probe for PCR DNA amplification techniques and DNA analysis in hybridization experiments, has been developed by researchers from RIKEN and ...