Grow the MEF cultures to confluency, collect the cells and freeze ... HESCs in p60 tissue culture dishes; other culturing vessels can be used 16, but it is advised that volumes are scaled up ...

1 The 100-cm2 growth area of each OptiCell (33% more than a T-75 flask) requires only 10 ml of growth medium. Building on this invention, BioCrystal developed a fully automated Robotic Cell Culture ...

While in vivo cells are protected by the immune system, there is no immune response in a tissue culture flask, so the cells are vulnerable to opportunistic pathogens and contaminants. Contamination ...