There are many popular chemistries for real-time PCR. One class uses different fluorescent dyes incorporated in short oligonucleotide probes specific for the amplified target. The second class ...

Subsequent to a final washing step, a PCR reagent mixture is added, containing specific TaqMan® probes that enable real-time PCR detection of the amplified DNA marker. 10,000-fold improved ...

Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

Expert Rev Mol Diagn. 2012;12(7):731-754. SYBR Green and dual-hybridization probes are often used for melting point analysis at the end of the amplification. qPCR presents a number of advantages ...

The GoTaq ® Probe 1-Step RT-qPCR System enables detection and relative ... control of possible carryover contamination. Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure ...

The DCI told the court the suspect's true identity remains in doubt and if released he is likely to abscond. But the Magistrate declined to grant them 14 days and instead gave them four days. CA ...